Facilities

stem-cells-training-researchers-human-iPSCs

The Center provides users with a clean, dedicated space specifically for culturing human induced pluripotent stem cells (iPSCs), occupying approximately 1,250 square feet of space on the second floor of Porter Biosciences, on the Â鶹Ãâ·Ñ°æÏÂÔØBoulder main campus.Ìý

We provide:

  • 5ÌýBiosafety Cabinets
  • 7 CO2Ìýand tri-gasÌýincubators
  • Automated Fluorescence Cell Counter
  • Inverted Fluorescence Scanner Microscope (Olympus IX83)Ìýwith live-cell time-lapse capabilitiesÌý(Live and fixed cell imaging in monolayer or suspensions, organoids, embryos, organotypic slides, and more!)
  • Sterile Dissection Hood equipped with a dissecting Olympus CKX53 microscope and heated/CO2Ìýstage for work with live cultures and live/fix imagine
  • OlympusÌýProviÌýCM20Ìý(imaging and monitoring cell confluence, cell count, and cell culture data records)
  • EVOS7000 Microscope
  • CO2 shaker + Remote Controller for Organoids Derivation
  • Transfection Systems (Neon NxT Electroporation &ÌýAmaxaÌýNucleofector)
  • Phase Contrast MicroscopesÌý
  • Liquid Nitrogen Cryostorage Space
  • 6 Water/Beads Bath andÌýThawSTARÌýAutomated Cell Thawing System
  • Centrifuges and Microcentrifuges
  • Plate shakers
  • Block heathers (microcentrifuge tubes)
  • Mr. FrostyÌý&ÌýCoolCellÌýÌýLX Cell Freezing Containers
  • Bioengineering SpaceÌý
  • Standard iPSC consumables (i.e., pipettes, plasticware, media, substrates, etc.) are readily available to users.Ìý
  • Reagents for sale (even 30% less)Ìý
  • Training of new users
  • hiPSC training. Ìý ***Coming soon: Neural Culture workshop!
  • Microscope training
  • Refrigerators &Ìýfreezers (4OC, -20oC, -80oC)

Stem cell center user using a microscope to inspect cells.

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